EGFR-Multiplex 5% AF cfDNA in Plasma

EGFR mutations are driver mutations, they are common and cause lung and breast cancer. The mutations of the SensID EGFR-Multiplex product consist of a selection of TKI sensitive and TKI resistant mutations, which are relevant for the therapy of the Non-Small Cell Lung Cancer.

The EGFR-Multiplex 5% AF cfDNA in plasma product can be used in diagnostics as a positive standard in liquid biopsy assays in your laboratory or R&D department, as well as for validation and development of EGFR diagnostic kits. The background material is human cfDNA of the Ashkenazim Son cell line, as well as human-tech plasma (human recreated).

SKU: SID-000018 Category:

Product description

Tailored to your needs: From bulk delivery and custom batch sizes to self-crafted with the corresponding cfDNA in buffer product!

OEM options:

If you need materials as in kit controls or as a third party control for validation of your kits at customer labs, please contact us via:

Self-Crafted Solution:

How to create your full workflow plasma control material:

If you prefer to make your own full workflow controls we recommend using our DNA depleted plasma products. Blend the DNA as necessary with the DNA depleted plasma to receive plasma materials with the necessary DNA concentrations.

This product as cfDNA in buffer can be found here:

Combine with:

And create your own plasma materials.

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method
– to control the extraction efficiency

Fragmentation Size (peak)
~167 bp

Unit Size: as desired


AA Change (Cosmic ID, mutation type, HGVS nomenclature, Exon)

  • p.G719S (COSV51767289*, Substitution, c.2155G>A, Exon 18)
  • p.E746_A750delELREA (COSV51765066*, Deletion, c.2236_2250del15, Exon 19)
  • p.S752_I759delSPANKEI (COSV51774879*, Deletion, c.2254_2277del24, Exon 19)
  • p.S768I (COSV51768106*, Substitution, c.2303G>T, Exon 20)
  • p.V769_D770insASV (COSV51850427*, Insertion, c.2303_2304ins9, Exon 20)
  • p.T790M (COSV51765492*, Substitution, c.2369C>T, Exon  20)
  • p.L858R (COSV51765161*, Substitution, c.2573T>G, Exon 21)
  • p.L861Q (COSV51766344 *, Substitution, c.2582T>A, Exon 21)

Allele frequency: 5%

Buffer: Plasma (human-tech)

Storage: 2-8 °C

Stable up to 24 months after date of manufacture (as supplied). In the case of in-house production, this depends on the manufacturing conditions

Quality control

Fragmentation size: High Sensitivity DNA Kit Agilent

Allele Frequency: dPCR


  • UV-Vis Spectrophotometry (NIST-Reference method)
  • dsDNA (fluorometric measurement)

Technical background

Derived from: cell line GM24385 (HG002- NA24385 – huAA53E0)

– lot specific sequencing files: LOT Search
– High-confidence variant calls:
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio:

*GRCh 38 Cosmic v88



Certificate of Analysis:

Batch Certificate

Lot: 00035

Lot: 00130

other Documents:

Instructions for use

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)

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