gDNA

SensIDs genomic DNA (gDNA) is a highly characterized human DNA from cell lines. Details on mutation status of specific genes and Whole Genome Sequencing (WGS) data are freely available through the Personal Genome Project (PGP) and our own quality assurance.

These products are ideal for Next Generation Sequencing (NGS) workflows. In particular:
- Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
- Calibration and development of instruments and workflows in DNA processing (e.g. DNA fragmentation via acoustic shearing, enzymatic digestion or sonication)
- Analyze the performance of your NGS pipeline by comparing to freely available datasets

SID – gDNA (human) wt

80,00

„SID – gDNA (human) wt“ is a highly characterized human DNA from cell lines. Details on mutation status of specific genes and Whole Genome Sequencing (WGS) data are freely available through the Personal Genome Project (PGP) and our own quality assurance.

SKU: SID-0004 Category:

Product description

These products are ideal for Next Generation Sequencing (NGS) workflows. In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Calibration and development of instruments and workflows in DNA processing (e.g. DNA fragmentation via acoustic shearing, enzymatic digestion or sonication)
– Analyze the performance of your NGS pipeline by comparing to freely available datasets

Unit Size:
5 µg

Concentration:
50 ng/µl

Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0

Storage:
2-8 °C

Expiry:
stable for 12 months from date of manufacture (as supplied)

Quality control

Allelic Frequency:
Next Generation Sequencing

Quantification:
UV-Vis Spectrophotometry (NIST-reference method)

Quality:
Agarose gel electrophoresis

Technical background

Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)

Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/

Documents

Certificate of Analysis:

Batch Certificate

other Documents:

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)

Instructions