EGFR-Multiplex 5% AF cfDNA

EGFR mutations are driver mutations, they are common and cause lung and breast cancer. The mutations of the SensID EGFR-Multiplex product consist of a selection of TKI sensitive and TKI resistant mutations, which are relevant for the therapy of the Non-Small Cell Lung Cancer.

The EGFR-Multiplex 5% AF cfDNA product can be used in diagnostics as a positive standard in liquid biopsy assays in your laboratory or R&D department, as well as for validation and development of EGFR diagnostic kits. Background material is highly characterized human cfDNA from cell lines.

SKU: SID-000019 Category:

Product description

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method

Fragmentation size (peak)
~167 bp

Unit Size:
400 ng

20 ng/µl


AA Change (Cosmic ID, Mutation type, HGVS Nomenklatur, Exon)

  • p.G719S (COSV51767289*, Substitution, c.2155G>A, Exon 18)
  • p.E746_A750delELREA (COSV51765066*, Deletion, c.2236_2250del15, Exon 19)
  • p.S752_I759delSPANKEI (COSV51774879*, Deletion, c.2254_2277del24, Exon 19)
  • p.S768I (COSV51768106*, Substitution, c.2303G>T, Exon 20)
  • p.V769_D770insASV (COSV51850427*, Insertion, c.2303_2304ins9, Exon 20)
  • p.T790M (COSV51765492*, Substitution, c.2369C>T, Exon  20)
  • p.L858R (COSV51765161*, Substitution, c.2573T>G, Exon 21)
  • p.L861Q (COSV51766344 *, Substitution, c.2582T>A, Exon 21)

Allelic frequency

Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0

2-8 °C

stable for 24 months from date of manufacture (as supplied)

Quality control

Fragmentation size:
High Sensitivity DNA Kit Agilent

Allelic Frequency:

UV-Vis Spectrophotometry (NIST-Reference method)
Qubit Fluorometry

Technical background

Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)

– lot specific sequencing files: LOT Search
– High-confidence variant calls:
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio:

*GRCh 38 Cosmic v88


Certificate of Analysis:

Batch Certificate

Lot: 00011

Lot: 00014

Lot: 00037

Lot: 00126

Lot: 00522

other Documents:

Instructions for use

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)

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