5-Gene-Multiplex Set cfDNA in Plasma, BRAF/KRAS/PIK3CA/AKT1/ERBB2; AF: 0%; 0.1%; 1%; 5%

The selected genes and mutations are diagnostically and therapeutically highly relevant and also play a role in various tumor entities:

Breast Carcinoma,  Colorectal Carcinoma,  Melanoma, Non-Small Cell Lung Carcinoma,  Small Cell Lung Carcinoma,  Prostate Carcinoma,  Lymphoma,  Head and Neck Carcinoma,  Hepatocellular Carcinoma,  Multiple Myeloma,  Pancreatic Adenocarcinoma,  Ovarian Carcinoma,  Bladder Carcinoma, Bile Duct Carcinoma and more.

The 5-Gene-Multiplex Set cfDNA in plasma product can be used in diagnostics as an allelic frequency standard range in liquid biopsy assays in your laboratory or R&D department, as well as for validation and development of diagnostic kits. Background material is highly characterized human cfDNA from cell lines. The set consists of the individual products SID-000002, SID-000088, SID-000089 and SID-000090 and can be used to validate your internal processes qualitatively and quantitatively with 0%, 0.1%, 1% and 5% allelic frequency.

SKU: SID-000091 Category:

Product description

Tailored to your needs: From bulk delivery and custom batch sizes to self-crafted with the corresponding cfDNA in buffer product!

OEM options:

If you need materials as in kit controls or as a third party control for validation of your kits at customer labs, please contact us via: sales@sens-id.com

Self-Crafted Solution:

How to create your full workflow plasma control material:

If you prefer to make your own full workflow controls we recommend using our DNA depleted plasma products. Blend the DNA as necessary with the DNA depleted plasma to receive plasma materials with the necessary DNA concentrations.

This product as cfDNA in buffer can be found here:

Combine with:

And create your own plasma materials.

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method
– to control the extraction efficiency

Unit Size: as desired


  • AKT1 – p.E17K (COSV62571334*, Substitution, c.49G>A)
  • BRAF – p.V600E (COSV56056643*, Substitution, c.1799T>A)
  • ERBB2 – p.Y772_A775dup (COSV54062409*, Insertion, c.2323_2324dup)
  • KRAS – p.G12D (COSV55497369*, Substitution, c.35G>A)
  • KRAS – p.Q61K (COSV55502066*, Substitution, c.181C>A)
  • KRAS – p.A146T (COSV55501778*, Substitution, c.436G>A)
  • PIK3CA – p.E545K (COSV55873239*, Substitution, c.1633G>A)
  • PIK3CA – p.H1047R (COSV55873195*, Substitution, c.3140A>G)

Allele frequencies: 0%; 0.1%; 1%; 5%

Buffer: Plasma (human-tech)

Storage: 2-8 °C

Stable up to 24 months after date of manufacture (as supplied). In the case of in-house production, this depends on the manufacturing conditions

Quality control

Fragmentation size: High Sensitivity DNA Kit Agilent

Allele Frequency: dPCR


  • UV-Vis Spectrophotometry (NIST-Reference method)
  • dsDNA (fluorometric measurement)

Technical background

Derived from: cell line GM24385 (HG002- NA24385 – huAA53E0)

– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/

*GRCh 38 Cosmic v88



Certificate of Analysis:

Batch Certificate

other Documents:

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)


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