Ashkenazim Son FFPE Reference Standard curl

Commutable FFPE Controls to mimic patient samples

Formalin Fixed Paraffin Embedded Reference Standards. This product is cell line derived.

This group of products can be used in diagnostics as a full workflow control for assays in your diagnostic laboratory or R&D department, as well as for validation and development of FFPE diagnostic or extraction kits. Ashkenazim son is specifically designed as a wildtype control for most cancer genes.

SKU: SID-000100 Category:

Product description

This quality control product is ideally suited as reference material in clinical genomics workflows. In particular:

– Validation and development of extraction protocols

– control in sequencing protocols (e.g. Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), Amplicon Sequencing) and PCR protocols

– Determination of the detectiont limit of a method

– Calibration and development of instruments and workflows in DNA processing (e.g. DNA fragmentation via acoustic shearing, enzymatic digestion or sonication)

– Analyze the performance of your NGS pipeline by comparing to freely available datasets

– routine performance monitoring (Research Use Only)

Product Overview

Format: FFPE (Formalin Fixed Paraffin Embedded)

Unit size: 5 FFPE curls

Quantity per vial: 1 FFPE curl

Expected DNA yield: ≥ 400 ng (using Qiagen AllPrep DNA/RNA FFPE kit)

Expected RNA yield: ≥ 400 ng (using Qiagen AllPrep DNA/RNA FFPE kit)

Thickness of one curl/cut: 10 µm


Wild type for most tumor relevant mutations. The genome was sequenced using various methods (see below) and tested orthogeneously for specific mutations using ddPCR.

Allelic frequency: No

Storage: 2-8 °C

Expiry: stable for 24 months from date of manufacturing (as supplied)

Quality Control (DNA)

Allelic Frequency (metrologically traceable): ddPCR (Bio-Rad)

Quantification (metrologically traceable): Fluorometric dsDNA measurement (Qubit)

DNA Quality: Agarose gel electrophoresis

Quality Control (RNA)

RNA Quantification: Fluorometric RNA measurement (Qubit)

Quality Control (other)

Cell Density:

1. Visual via microscopy

2. Calibrated measurement

Technical Background

Derived from:
The Genome In A Bottle (GIAB) cell line from the Personal Genome Project (PGB): GM24385 (HG002- NA24385 – huAA53E0 – Ashkenazim son)

– Lot specific sequencing files: LOT Search
– High-confidence variant calls:
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio:

* GRCh38 · COSMIC v90


Certificate of Analysis:

Batch Certificate

Lot: 00060

Lot: 00254

Lot: 00531

Other documents:

Instructions for use

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)

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