EGFR-Multiplex 0.1% AF cfDNA

EGFR mutations are driver mutations, they are common and cause lung and breast cancer. The mutations of the SensID EGFR-Multiplex product consist of a selection of TKI sensitive and TKI resistant mutations, which are relevant for the therapy of the Non-Small Cell Lung Cancer.

The EGFR-Multiplex 0,1% AF cfDNA product can be used in diagnostics as a positive standard in liquid biopsy assays in your laboratory or R&D department, as well as for validation and development of EGFR diagnostic kits. Background material is highly characterized human cfDNA from cell lines.

SKU: SID-000015 Category:

Product description

Also available as a customized product tailored to your needs!

OEM options:

If you need materials as in kit controls or as a third-party control for validation of your kits at customer labs, please contact us via:

How to create your full workflow plasma control material based on this product:

We recommend the use of our human DNA-free plasma products to produce complete workflow controls with this product. Blend the cfDNA as necessary with the DNA depleted plasma to receive plasma materials with the necessary DNA concentrations.

Choose your plasma volume options :

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method

Unit Size:
400 ng

Fragmentation size:
~167 bp

20 ng/µl


AA Change (Cosmic ID, mutation type, HGVS nomenclature, Exon)

  • p.G719S (COSV51767289*, Substitution, c.2155G>A, Exon 18)
  • p.E746_A750delELREA (COSV51765066*, Deletion, c.2236_2250del15, Exon 19)
  • p.S752_I759delSPANKEI (COSV51774879*, Deletion, c.2254_2277del24, Exon 19)
  • p.S768I (COSV51768106*, Substitution, c.2303G>T, Exon 20)
  • p.V769_D770insASV (COSV51850427*, Insertion, c.2303_2304ins9, Exon 20)
  • p.T790M (COSV51765492*, Substitution, c.2369C>T, Exon  20)
  • p.L858R (COSV51765161*, Substitution, c.2573T>G, Exon 21)
  • p.L861Q (COSV51766344 *, Substitution, c.2582T>A, Exon 21)

Allele frequency

Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0

2-8 °C

stable for 24 months from date of manufacture (as supplied)

Quality control

Fragmentation size:
High Sensitivity DNA Kit Agilent

Allele Frequency:

UV-Vis Spectrophotometry (NIST-Reference method)
Qubit Fluorometry

Technical background

Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)

– lot specific sequencing files: LOT Search
– High-confidence variant calls:
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio:

*GRCh 38 Cosmic v88


Certificate of Analysis:

Batch Certificate

Lot: 00009

Lot: 00012

Lot: 00082

Lot: 00520

other Documents:

Instructions for use

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)

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