EGFR-Multiplex 0,1% AF cfDNA

EGFR mutations are driver mutations, they are common and cause lung and breast cancer. The mutations of the SensID EGFR-Multiplex product consist of a selection of TKI sensitive and TKI resistant mutations, which are relevant for the therapy of the Non-Small Cell Lung Cancer.

The EGFR-Multiplex 0,1% AF cfDNA product can be used in diagnostics as a positive standard in liquid biopsy assays in your laboratory or R&D department, as well as for validation and development of EGFR diagnostic kits. Background material is highly characterized human cfDNA from cell lines.

SKU: SID-000015 Category:

Product description

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method

Unit Size:
400 ng

Concentration:
20 ng/µl

Mutations:

AA Change (Cosmic ID, mutation type, HGVS nomenclature, Exon)

  • p.G719S (COSM6252*, substitution, c.2155G>A, Exon 18)
  • p.E746_A750delELREA (COSM6225*, deletion, c.2236_2250del15, Exon 19)
  • p.S752_I759delSPANKEI (COSM6256*, deletion, c.2254_2277del24, Exon 19)
  • p.S768I (COSM6241*, substitution, c.2303G>T, Exon 20)
  • p.V769_D770insASV (COSM20884*, insertion, c.2303_2304ins9, Exon 20)
  • p.T790M (COSM6240*, substitution, c.2369C>T, Exon  20)
  • p.L858R (COSM6224*, substitution, c.2573T>G, Exon 21)
  • p.L861Q (COSM6213*, substitution, c.2582T>A, Exon 21)

Allelic frequency
0,1%

Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0

Storage:
2-8 °C

Expiry:
stable for 12 months from date of manufacture (as supplied)

Quality control

Fragmentation size:
High Sensitivity DNA Kit Agilent

Allelic Frequency:
dPCR

Quantification:
UV-Vis Spectrophotometry (NIST-Reference method)

Technical background

Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)

Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/

*GRCh 38 Cosmic v88

Documents

Certificate of Analysis:

Batch Certificate


Lot: 00009

other Documents:

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)

Instructions