EGFR-Multiplex Set cfDNA

EGFR mutations are driver mutations, they are common and cause lung and breast cancer. The mutations of the SensID EGFR-Multiplex product consist of a selection of TKI sensitive and TKI resistant mutations, which are relevant for the therapy of the Non-Small Cell Lung Cancer.

The EGFR-Multiplex Set cfDNA product can be used in diagnostics as a allelic frequency standard range in liquid biopsy assays in your laboratory or R&D department, as well as for validation and development of EGFR diagnostic kits. Background material is highly characterized human cfDNA from cell lines. The set consists of the individual products SID-0003, SID-000015, SID-000017 and SID-000019, so it can be used to validate your internal processes qualitatively and quantitatively with 0%, 0.1%, 1% and 5% allelic frequency.

SKU: SID-000021 Category:

Product description

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method

Unit Size:
400 ng

20 ng/µl


AA Change (Cosmic ID, mutation type, HGVS nomenclature, Exon)

  • p.G719S (COSM6252*, substitution, c.2155G>A, Exon 18)
  • p.E746_A750delELREA (COSM6225*, deletion, c.2236_2250del15, Exon 19)
  • p.S752_I759delSPANKEI (COSM6256*, deletion, c.2254_2277del24, Exon 19)
  • p.S768I (COSM6241*, substitution, c.2303G>T, Exon 20)
  • p.V769_D770insASV (COSM20884*, insertion, c.2303_2304ins9, Exon 20)
  • p.T790M (COSM6240*, substitution, c.2369C>T, Exon  20)
  • p.L858R (COSM6224*, substitution, c.2573T>G, Exon 21)
  • p.L861Q (COSM6213*, substitution, c.2582T>A, Exon 21)

Allelic frequency
0%, 0,1%, 1% and 5%

Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0

2-8 °C

stable for 12 months from date of manufacture (as supplied)

Quality control

Fragmentation size:
High Sensitivity DNA Kit Agilent

Allelic Frequency:

UV-Vis Spectrophotometry (NIST-Reference method)

Technical background

Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)

– lot specific sequencing files: LOT Search
– High-confidence variant calls:
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio:

*GRCh 38 Cosmic v88


Certificate of Analysis:

Batch Certificate


other Documents:

Safety Data Sheet (Available on request. Please contact the support or see the product pages for the individual components of the set.)