EGFR-T790M 1% AF cfDNA in Plasma

EGFR mutations are driver mutations, they are common and cause lung and breast cancer. A mutation in the epidermal growth factor receptor (EGFR) is the T790M mutation, which is relevant for therapy.

The EGFR T790M 1% AF cfDNA in plasma product can be used in diagnostics as a positive standard in liquid biopsy assays in your laboratory or R&D department, as well as for validation and development of EGFR diagnostic kits. The background material is human cfDNA of the Ashkenazim Son cell line, as well as human-tech plasma (human recreated).

SKU: SID-000012 Category:

Product description

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Determination of the detectiont limit of the method
– to control the extraction efficiency

Fragmentation Size (peak)
~167 bp

Unit Size:
80 ng/ml (total 400 ng)


  • AA Change  p.T790M
  • Cosmic ID  COSV51765492*
  • Mutation type  substitution
  • HGVS Nomenklatur  c.2369C>T
  • Exon  20

Allelic frequency:

Plasma (human-tech)

2-8 °C

stable for 12 months from date of manufacture (as supplied)

Quality control

Fragmentation size:
High Sensitivity DNA Kit Agilent

Allelic Frequency:

UV-Vis Spectrophotometry (NIST-Reference method)
Qubit Fluorometry

Technical background

Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)

– lot specific sequencing files: LOT Search
– High-confidence variant calls:
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio:

*GRCh 38 Cosmic v88


Certificate of Analysis:

Batch Certificate

Lot: 00007

Lot: 00051

Lot: 00077

Lot: 00079

other Documents:

Instructions for use

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)