SID – cfDNA (human) wt

90,00

SID – cfDNA (human) wt is a highly characterized human DNA from cell lines. Manufactured for liquid biopsy assays in your laboratory or R&D department. Details on mutation status of specific genes and Whole Genome Sequencing (WGS) data are freely available through the Personal Genome Project (PGP) and our own quality assurance.

SKU: SID-0003 Category:

Product description

These products are ideal for digital PCR and/or Next Generation Sequencing (NGS). In particular:
– Validation and development of sequencing protocols (e.g. Whole Genome Sequencing (WGS), Amplicon Sequencing) and PCR protocols
– Analyze the performance of your NGS pipeline by comparing to freely available datasets

Unit Size:
400 ng

Concentration:
20 ng/µl

Buffer:
Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8,0

Storage:
2-8 °C

Expiry:
stable for 12 months from date of manufacture (as supplied)

Quality control

Fragmentation size:
High Sensitivity DNA Kit Agilent

Allelic Frequency:
Next Generation Sequencing

Quantification:
UV-Vis Spectrophotometry (NIST-Reference method)

Technical background

Derived from:
cell line GM24385 (HG002- NA24385 – huAA53E0)

Bioinformatics:
– lot specific sequencing files: LOT Search
– High-confidence variant calls: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/release/AshkenazimTrio
– Raw datasets and bam files, currently including 10X Genomics, BioNano, Complete Genomics regular and LFR, 300x Illumina paired-end, Illumina 6kb mate-pair, 1000x Ion exome, custom moleculo libraries, ~0.05x Oxford Nanopore, and 70x/30x/30x PacBio: ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/

Documents

Certificate of Analysis:

Batch Certificate

other Documents:

Safety Data Sheet (SDS in other languages ​​available on request. Please contact the support.)

Instructions